{"id":2379,"date":"2019-10-02T20:06:00","date_gmt":"2019-10-02T20:06:00","guid":{"rendered":"https:\/\/scian.cl\/scientific-image-analysis\/?p=2379"},"modified":"2023-05-31T01:27:14","modified_gmt":"2023-05-31T01:27:14","slug":"cdh2-coordinates-myosin-ii-dependent-internalisation-of-the-zebrafish-neural-plate","status":"publish","type":"post","link":"https:\/\/scian.cl\/scientific-image-analysis\/cdh2-coordinates-myosin-ii-dependent-internalisation-of-the-zebrafish-neural-plate\/","title":{"rendered":"Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate"},"content":{"rendered":"\n<p class=\"has-medium-font-size\"><em><span class=\"has-inline-color has-theme-palette-6-color\">Scientific Reports, 9(1):1835 (2019)<\/span><\/em><\/p>\n\n\n\n<p>Araya C, Hakkinen H, Carcamo L, Cerda M, Savy T, Rookyard C, Peyri\u00e9ras N, Clarke J.<\/p>\n\n\n\n<p><strong>ABSTRACT<\/strong><br>Tissue internalisation is a key morphogenetic mechanism by which embryonic tissues generate complex internal organs and a number of studies of epithelia have outlined a general view of tissue internalisation. Here we have used quantitative live imaging and mutant analysis to determine whether similar mechanisms are responsible for internalisation in a tissue that apparently does not have a typical epithelial organisation \u2013 the zebrafish neural plate. We found that although zebrafish embryos begin neurulation without a conventional epithelium, medially located neural plate cells adopt strategies typical of epithelia in order to constrict their dorsal surface membrane during cell internalisation. Furthermore, we show that Myosin-II activity is a significant driver of this transient cell remodeling which also depends on Cdh2 (N-cadherin). Abrogation of Cdh2 results in defective Myosin-II distribution, mislocalised internalisation events and defective neural plate morphogenesis. Our work suggests Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate.<\/p>\n\n\n\n<p>LINK:<br><a href=\"https:\/\/doi.org\/10.1038\/s41598-018-38455-w\">https:\/\/doi.org\/10.1038\/s41598-018-38455-w<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Araya, C., H\u00e4kkinen, H. M., Carcamo, L., Cerda, M., Savy, T., Rookyard, C., &#8230; &#038; Clarke, J. D. (2019). Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate. Scientific Reports, 9(1), 1835.<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[23],"tags":[],"class_list":["post-2379","post","type-post","status-publish","format-standard","hentry","category-publications-2019"],"_links":{"self":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2379","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/comments?post=2379"}],"version-history":[{"count":3,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2379\/revisions"}],"predecessor-version":[{"id":4028,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2379\/revisions\/4028"}],"wp:attachment":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/media?parent=2379"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/categories?post=2379"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/tags?post=2379"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}