{"id":2474,"date":"2015-10-03T22:11:00","date_gmt":"2015-10-03T22:11:00","guid":{"rendered":"https:\/\/scian.cl\/scientific-image-analysis\/?p=2474"},"modified":"2023-06-17T02:07:31","modified_gmt":"2023-06-17T02:07:31","slug":"transactivation-activity-and-nucleocytoplasmic-transport-of-catenin-are-independently-regulated-by-its-c-terminal-end","status":"publish","type":"post","link":"https:\/\/scian.cl\/scientific-image-analysis\/transactivation-activity-and-nucleocytoplasmic-transport-of-catenin-are-independently-regulated-by-its-c-terminal-end\/","title":{"rendered":"Transactivation activity and nucleocytoplasmic transport of <beta>-catenin are independently regulated by its C-terminal end"},"content":{"rendered":"\n<p>Maturana J, Niechi I, Silva-Pavez E, Huerta-Castro H, Cataldo R, H\u00e4rtel S, Barros F, Galindo M &amp; J Tapia<br>Gene 573(1), 115\ufffd122. http:\/\/dx.doi.org\/10.1016\/j.gene.2015.07.039<\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-_ef1db0-b3 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-_ef1db0-b3 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-_ef1db0-b3\"><div class=\"kt-block-spacer kt-block-spacer-halign-left\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<p><strong>ABSTRACT<\/strong><br>The key protein in the canonicalWnt pathway is \u03b2-catenin, which is phosphorylated both in absence and presence ofWnt signals by different kinases. Upon activation in the cytoplasm, \u03b2-catenin can enter into the nucleus to transactivate target gene expression, many of which are cancer-related genes. The mechanism governing \u03b2- catenin&#8217;s nucleocytoplasmic transport has been recently unvealed, although phosphorylation at its C-terminal end and its functional consequences are not completely understood. Serine 646 of \u03b2-catenin is a putative CK2 phosphorylation site and lies in a region which has been proposed to be important for its nucleocytoplasmic transport and transactivation activity. This residue was mutated to aspartic acid mimicking CK2- phosphorylation and its effects on \u03b2-catenin activity as well as localization were explored. \u03b2-Catenin S6464D did not show significant differences in both transcriptional activity and nuclear localization compared to the wild-type form, but displayed a characteristic granular nuclear pattern. Three-dimensional models of nuclei were constructed which showed differences in number and volume of granules, being those from \u03b2-catenin S646D more and smaller than the wild-type form. FRAP microscopy was used to compare nuclear export of both proteins which showed a slightly higher but not significant retention of \u03b2-catenin S646D. Altogether, these results show that C-terminal phosphorylation of \u03b2-catenin seems to be related with its nucleocytoplasmic transport but not transactivation activity.<\/p>\n\n\n\n<p><a href=\"https:\/\/scian.cl\/scientific-image-analysis\/wp-content\/uploads\/2021\/10\/2015_Maturana-JL_Gene_SHartel.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Download PDF<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Maturana, J. L., Niechi, I., Silva, E., Huerta, H., Cataldo, R., H\u00e4rtel, S., &#8230; &#038; Tapia, J. C. (2015). Transactivation activity and nucleocytoplasmic transport of \u03b2-catenin are independently regulated by its C-terminal end. Gene, 573(1), 115-122.<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[27],"tags":[],"class_list":["post-2474","post","type-post","status-publish","format-standard","hentry","category-publication-2015"],"_links":{"self":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2474","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/comments?post=2474"}],"version-history":[{"count":3,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2474\/revisions"}],"predecessor-version":[{"id":4146,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2474\/revisions\/4146"}],"wp:attachment":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/media?parent=2474"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/categories?post=2474"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/tags?post=2474"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}