{"id":2553,"date":"2012-10-02T23:36:00","date_gmt":"2012-10-02T23:36:00","guid":{"rendered":"https:\/\/scian.cl\/scientific-image-analysis\/?p=2553"},"modified":"2023-06-17T03:00:12","modified_gmt":"2023-06-17T03:00:12","slug":"caveolin-1-enhanced-motility-and-focal-adhesion-turnover-require-tyrosine-14-but-not-accumulation-to-the-rear-in-metastatic-cancer-cells","status":"publish","type":"post","link":"https:\/\/scian.cl\/scientific-image-analysis\/caveolin-1-enhanced-motility-and-focal-adhesion-turnover-require-tyrosine-14-but-not-accumulation-to-the-rear-in-metastatic-cancer-cells\/","title":{"rendered":"Caveolin-1-Enhanced Motility and Focal Adhesion Turnover Require Tyrosine-14 but not Accumulation to the Rear in Metastatic Cancer Cells"},"content":{"rendered":"\n<p>Urra H, Torres V, Ortiz R, Lobos L, D\u00edaz M, D\u00edaz N, H\u00e4rtel S, Leyton L, and A Quest<br>PLosOne, Volume 7, Issue 4, e33085, 1-14<\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-_eba008-64 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-_eba008-64 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-_eba008-64\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<p><strong>ABSTRACT<\/strong><br>Caveolin-1 is known to promote cell migration, and increased caveolin-1 expression is associated with tumor progression and metastasis. In fibroblasts, caveolin-1 polarization and phosphorylation of tyrosine-14 are essential to promote migration. However, the role of caveolin-1 in migration of metastatic cells remains poorly defined. Here, caveolin-1 participation in metastatic cell migration was evaluated by shRNA targeting of endogenous caveolin-1 in MDA-MB-231 human breast cancer cells and ectopic expression in B16-F10 mouse melanoma cells. Depletion of caveolin-1 in MDA-MB-231 cells reduced, while expression in B16-F10 cells promoted migration, polarization and focal adhesion turnover in a sequence of events that involved phosphorylation of tyrosine-14 and Rac-1 activation. In B16-F10 cells, expression of a non-phosphorylatable tyrosine-14 to phenylalanine mutant failed to recapitulate the effects observed with wild-type caveolin-1. Alternatively, treatment of MDA-MB-231 cells with the Src family kinase inhibitor PP2 reduced caveolin-1 phosphorylation on tyrosine-14 and cell migration. Surprisingly, unlike for fibroblasts, caveolin-1 polarization and re-localization to the trailing edge were not observed in migrating metastatic cells. Thus, expression and phosphorylation, but not polarization of caveolin-1 favor the highly mobile phenotype of metastatic cells.<\/p>\n\n\n\n<p><a href=\"https:\/\/scian.cl\/scientific-image-analysis\/wp-content\/uploads\/2021\/10\/2012_PlosOne_shartel.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Download PDF<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Urra, H., Torres, V. A., Ortiz, R. J., Lobos, L., D\u00edaz, M. I., D\u00edaz, N., &#8230; &#038; Quest, A. F. (2012). Caveolin-1-enhanced motility and focal adhesion turnover require tyrosine-14 but not accumulation to the rear in metastatic cancer cells. PloS one, 7(4), e33085.<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[30],"tags":[],"class_list":["post-2553","post","type-post","status-publish","format-standard","hentry","category-publications-2012"],"_links":{"self":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2553","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/comments?post=2553"}],"version-history":[{"count":2,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2553\/revisions"}],"predecessor-version":[{"id":4183,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2553\/revisions\/4183"}],"wp:attachment":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/media?parent=2553"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/categories?post=2553"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/tags?post=2553"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}