{"id":2582,"date":"2011-10-01T00:05:00","date_gmt":"2011-10-01T00:05:00","guid":{"rendered":"https:\/\/scian.cl\/scientific-image-analysis\/?p=2582"},"modified":"2023-06-17T02:57:44","modified_gmt":"2023-06-17T02:57:44","slug":"high-frequency-field-stimulation-of-primary-neurons-enhances-ryanodine-receptor-mediated-ca2-release-and-generates-hydrogen-peroxide-which-jointly-stimulate-nf-kb-activity","status":"publish","type":"post","link":"https:\/\/scian.cl\/scientific-image-analysis\/high-frequency-field-stimulation-of-primary-neurons-enhances-ryanodine-receptor-mediated-ca2-release-and-generates-hydrogen-peroxide-which-jointly-stimulate-nf-kb-activity\/","title":{"rendered":"High-frequency Field Stimulation of Primary Neurons Enhances Ryanodine Receptor-mediated Ca2+ Release and Generates Hydrogen Peroxide, which Jointly Stimulate NF-kB Activity"},"content":{"rendered":"\n<p>Riquelme D, Alvarez A, Leal N, Adasme T, Espinoza I, Vald\u00e9s JA, Troncoso N, H\u00e4rtel S, Hidalgo J, Hidalgo C, &amp; MA Carrasco<br>Antioxid Redox Signal. 14(7):1245-59<\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-_e15180-d5 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-_e15180-d5 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-_e15180-d5\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<p><strong>ABSTRACT<\/strong><br>Abstract Neuronal electrical activity increases intracellular Ca(2+) concentration and generates reactive oxygen species. Here, we show that high frequency field stimulation of primary hippocampal neurons generated Ca(2+) signals with an early and a late component, and promoted hydrogen peroxide generation via a neuronal NADPH oxidase. Hydrogen peroxide generation required both Ca(2+) entry through N-methyl-D-aspartate receptors and Ca(2+) release mediated by ryanodine receptors (RyR). Field stimulation also enhanced nuclear translocation of the NF-\u03baB p65 protein and NF-\u03baB -dependent transcription, and increased c-fos mRNA and type-2 RyR protein content. Preincubation with inhibitory ryanodine or with the antioxidant N-acetyl L-cysteine abolished the increase in hydrogen peroxide generation and the late Ca(2+) signal component induced by electrical stimulation. Primary cortical cells behaved similarly as primary hippocampal cells. Exogenous hydrogen peroxide also activated NF-\u03baB-dependent transcription in hippocampal neurons; inhibitory ryanodine prevented this effect. Selective inhibition of the NADPH oxidase or N-acetyl L-cysteine also prevented the enhanced translocation of p65 in hippocampal cells, while N-acetyl L-cysteine abolished the increase in RyR2 protein content induced by high frequency stimulation. In conclusion, the present results show that electrical stimulation induced reciprocal activation of ryanodine receptor-mediated Ca(2+) signals and hydrogen peroxide generation, which stimulated jointly NF-\u03baB activity.<\/p>\n\n\n\n<p>Acknowledgments This work was supported by Fondo Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica (FONDECYT) grant 1060177 (MAC), by FONDAP Center for Molecular Studies of the Cell grant 15010006 (CH), and by Enlace FONDECYT-VID ENL 09\/02 (MAC). SCIAN-Lab is a member of the German-Chilean Center of Excellence Initiative for Medical Informatics (DAAD) and the Advanced Imaging &amp; Bioinformatics Initiative AI\u00b7BI (www.aibi.cl. Research in SCIAN-Lab (SH) is funded by FONDECYT 1090246, FONDEF (D07I1019), and ICM-P04-068-F (NEMO).<\/p>\n\n\n\n<p><a href=\"https:\/\/scian.cl\/scientific-image-analysis\/wp-content\/uploads\/2021\/10\/2011_AntioxidRedoxSignal_shartel.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Download PDF<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Riquelme, D., Alvarez, A., Leal, N., Adasme, T., Espinoza, I., Vald\u00e9s, J. A., &#8230; &#038; Carrasco, M. A. (2011). High-frequency field stimulation of primary neurons enhances ryanodine receptor-mediated Ca2+ release and generates hydrogen peroxide, which jointly stimulate NF-\u03baB activity. Antioxidants &#038; redox signaling, 14(7), 1245-1259.<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[31],"tags":[],"class_list":["post-2582","post","type-post","status-publish","format-standard","hentry","category-publications-2011"],"_links":{"self":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2582","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/comments?post=2582"}],"version-history":[{"count":2,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2582\/revisions"}],"predecessor-version":[{"id":4181,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2582\/revisions\/4181"}],"wp:attachment":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/media?parent=2582"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/categories?post=2582"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/tags?post=2582"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}