{"id":2628,"date":"2007-10-03T01:06:00","date_gmt":"2007-10-03T01:06:00","guid":{"rendered":"https:\/\/scian.cl\/scientific-image-analysis\/?p=2628"},"modified":"2023-06-17T02:37:01","modified_gmt":"2023-06-17T02:37:01","slug":"marlin-1-and-conventional-kinesin-link-gabab-receptors-to-the-cytoskeleton-and-regulate-receptor-transport","status":"publish","type":"post","link":"https:\/\/scian.cl\/scientific-image-analysis\/marlin-1-and-conventional-kinesin-link-gabab-receptors-to-the-cytoskeleton-and-regulate-receptor-transport\/","title":{"rendered":"Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport."},"content":{"rendered":"\n<p>Vidal RL., Ram\u00edrez OA., Sandoval L., Koenig-Robert R., H\u00e4rtel S. and Couve A.<br>Molecular and Cellular Neuroscience, 35(3):501-12.<\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-_297f6f-59 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-_297f6f-59 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-_297f6f-59\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<p><strong>ABSTRACT<\/strong><br>The cytoskeleton and cytoskeletal motors play a fundamental role in neurotransmitter receptor trafficking, but proteins that link GABAB receptors (GABABRs) to the cytoskeleton have not been described.We recently identified Marlin-1, a protein that interacts with GABABR1. Here, we explore the association of GABABRs and Marlin-1 to the cytoskeleton using a combination of biochemistry, microscopy and live cell imaging. Our results indicate that Marlin-1 is associated to microtubules and the molecular motor kinesin-I. We demonstrate that a fraction of Marlin-1 is mobile in dendrites of cultured hippocampal neurons and that mobility is microtubule-dependent. We also show that GABABRs interact robustly with kinesin-I and that intracellular membranes containing GABABRs are sensitive to treatments that disrupt a protein complex containing Marlin-1, kinesin-I and tubulin. Finally, we report that a kinesin-I mutant severely impairs receptor transport. We conclude that Marlin-1 and kinesin-1 link GABABRs to the tubulin cytoskeleton in neurons.<\/p>\n\n\n\n<p><a href=\"https:\/\/scian.cl\/scientific-image-analysis\/wp-content\/uploads\/2021\/10\/2007_MolCelNeuroscience_shartel.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Download PDF<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Vidal, R. L., Ram\u00edrez, O. A., Sandoval, L., Koenig-Robert, R., H\u00e4rtel, S., &#038; Couve, A. (2007). Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport. Molecular and Cellular Neuroscience, 35(3), 501-512.<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[35],"tags":[],"class_list":["post-2628","post","type-post","status-publish","format-standard","hentry","category-publications-2007"],"_links":{"self":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2628","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/comments?post=2628"}],"version-history":[{"count":2,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2628\/revisions"}],"predecessor-version":[{"id":4158,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2628\/revisions\/4158"}],"wp:attachment":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/media?parent=2628"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/categories?post=2628"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/tags?post=2628"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}