{"id":2632,"date":"2007-10-01T01:10:00","date_gmt":"2007-10-01T01:10:00","guid":{"rendered":"https:\/\/scian.cl\/scientific-image-analysis\/?p=2632"},"modified":"2023-06-17T02:38:21","modified_gmt":"2023-06-17T02:38:21","slug":"3d-morpho-topological-analysis-of-asymmetric-neuronal-morphogenesis-in-developing-zebrafish-computational-modelling-of-objects-represented-in-images-fundamentals-methods-and-applications","status":"publish","type":"post","link":"https:\/\/scian.cl\/scientific-image-analysis\/3d-morpho-topological-analysis-of-asymmetric-neuronal-morphogenesis-in-developing-zebrafish-computational-modelling-of-objects-represented-in-images-fundamentals-methods-and-applications\/","title":{"rendered":"3D Morpho-Topological Analysis of Asymmetric Neuronal Morphogenesis in Developing Zebrafish. Computational Modelling of Objects Represented in Images. Fundamentals, Methods and Applications."},"content":{"rendered":"\n<p>S. H\u00e4rtel, J. Jara, C.G. Lemus, M.L. Concha<br>Ed. Jo\u00e3o Manuel Tavares &amp; Jorge Nata, Taylor and Francis Group, ISBN: 9780415433495, pp: 215-220.<\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-_c4337f-cc .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-_c4337f-cc .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-_c4337f-cc\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<p><strong>ABSTRACT<\/strong><br>We applied in vivo confocal microscopy of GFP-transgenic zebrafish in combination with 3D image analyses to study the asymmetric morphogenesis of the diencephalic parapineal organ on a supra-cellular, cellular, and sub-cellular level. Following a rough manual segmentation of the respective regions of interest (ROIs), the morphology of generated surface meshes was refined by an active surface model which it-eratively adjusts the mesh towards the morphology of the cellular structures. This procedure is essential for a precise morpho-topological analysis, mostly because of the adversarial diffraction limited resolution in the z-dimension of confocal image stacks. 3D Morphology and topology of the reconstructed cellular and supra-cellular structures during morphogenesis was quantified by principal axis transformations and 3D moment in-variants. Our data indicates that migration of the parapineal organ is accompanied by a rapid transition be-tween predominantly parallel cell orientations towards predominantly perpendicular orientations, a phenome-non which requires a precise control of cell shape and polarity. The orientational transition is followed by a phase of polarized cell motility in which membrane protrusions in the form of blebs and filopodia become ori-ented in the direction of the asymmetric migration. The morpho-topological descriptors unveil information that is not perceptible for a direct visual analysis of the microscopical data sets. This approach becomes essen-tial to access morphogenetic mechanisms which control asymmetry and migration.<\/p>\n\n\n\n<p><a href=\"https:\/\/scian.cl\/scientific-image-analysis\/wp-content\/uploads\/2021\/10\/2006_FullPaper_Coimbra_shartel.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Download PDF<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>H\u00e4rtel, S., Jara, J., Lemus, C. G., &#038; Concha, M. L. (2018). 3D morpho-topological analysis of asymmetric neuronal morphogenesis in developing zebrafish. In Computational modelling of objects represented in images (pp. 215-220). CRC Press.<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[35],"tags":[],"class_list":["post-2632","post","type-post","status-publish","format-standard","hentry","category-publications-2007"],"_links":{"self":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2632","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/comments?post=2632"}],"version-history":[{"count":2,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2632\/revisions"}],"predecessor-version":[{"id":4160,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2632\/revisions\/4160"}],"wp:attachment":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/media?parent=2632"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/categories?post=2632"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/tags?post=2632"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}