{"id":2642,"date":"2006-10-01T02:16:00","date_gmt":"2006-10-01T02:16:00","guid":{"rendered":"https:\/\/scian.cl\/scientific-image-analysis\/?p=2642"},"modified":"2023-06-17T02:35:45","modified_gmt":"2023-06-17T02:35:45","slug":"atp-steal-between-cation-pumps-a-mechanism-linking-na-influx-to-the-onset-of-necrotic-ca2-overload","status":"publish","type":"post","link":"https:\/\/scian.cl\/scientific-image-analysis\/atp-steal-between-cation-pumps-a-mechanism-linking-na-influx-to-the-onset-of-necrotic-ca2-overload\/","title":{"rendered":"ATP steal between cation pumps: a mechanism linking Na+ influx to the onset of necrotic Ca2+ overload."},"content":{"rendered":"\n<p>Castro J, Ruminot I, Porras OH, Flores CM, Hermosilla T, Verdugo E, Venegas F, Hartel S, Michea L &amp; Barros LF.<br>Cell Death &amp; Differentiation 13, pp:1675\ufffd1685.<\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-_c42bc1-d1 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-_c42bc1-d1 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-_c42bc1-d1\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<p><strong>ABSTRACT<\/strong><br>We set out to identify molecular mechanisms underlying the onset of necrotic Ca2+ overload, triggered in two epithelial cell lines by oxidative stress or metabolic depletion. As reported earlier, the overload was inhibited by extracellular Ca2+ chelation and the cation channel blocker gadolinium. However, the surface permeability to Ca2+ was reduced by 60%, thus discarding a role for Ca2+ channel\/carrier activation. Instead, we registered a collapse of the plasma membrane Ca2+ ATPase (PMCA). Remarkably, inhibition of the Na+\/K+ ATPase rescued the PMCA and reverted the Ca2+ rise. Thermodynamic considerations suggest that the Ca2+ overload develops when the Na+\/K+ ATPase, by virtue of the Na+ overload, clamps the ATP phosphorylation potential below the minimum required by the PMCA. In addition to providing the mechanism for the onset of Ca2+ overload, the crosstalk between cation pumps offers a novel explanation for the role of Na+ in cell death.<\/p>\n\n\n\n<p><a href=\"https:\/\/scian.cl\/scientific-image-analysis\/wp-content\/uploads\/2021\/10\/2006_CellDeathDifferentiation_shaertel.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Download PDF<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Castro, J., Ruminot, I., Porras, O. H., Flores, C. M., Hermosilla, T., Verdugo, E., &#8230; &#038; Barros, L. F. (2006). ATP steal between cation pumps: a mechanism linking Na+ influx to the onset of necrotic Ca2+ overload. Cell Death &#038; Differentiation, 13(10), 1675-1685.<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[36],"tags":[],"class_list":["post-2642","post","type-post","status-publish","format-standard","hentry","category-publications-2006"],"_links":{"self":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2642","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/comments?post=2642"}],"version-history":[{"count":2,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2642\/revisions"}],"predecessor-version":[{"id":4157,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/2642\/revisions\/4157"}],"wp:attachment":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/media?parent=2642"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/categories?post=2642"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/tags?post=2642"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}