{"id":3597,"date":"2021-11-24T19:56:44","date_gmt":"2021-11-24T19:56:44","guid":{"rendered":"https:\/\/scian.cl\/scientific-image-analysis\/?p=3597"},"modified":"2021-11-24T20:21:54","modified_gmt":"2021-11-24T20:21:54","slug":"ryanodine-receptor-mediated-ca2-release-and-atlastin-2-gtpase-activity-contribute-to-ip3-induced-dendritic-ca2-signals-in-primary-hippocampal-neurons-2","status":"publish","type":"post","link":"https:\/\/scian.cl\/scientific-image-analysis\/ryanodine-receptor-mediated-ca2-release-and-atlastin-2-gtpase-activity-contribute-to-ip3-induced-dendritic-ca2-signals-in-primary-hippocampal-neurons-2\/","title":{"rendered":"Ryanodine receptor-mediated Ca2+ release and atlastin-2 GTPase activity contribute to IP3-induced dendritic Ca2+ signals in primary hippocampal neurons"},"content":{"rendered":"\n<p class=\"has-medium-font-size\"><em><span class=\"has-inline-color has-theme-palette-6-color\">Cell Calcium<\/span><\/em><\/p>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-_f8b914-00 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-_f8b914-00 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-_f8b914-00\"><div class=\"kt-block-spacer kt-block-spacer-halign-left\" style=\"height:60px\"><hr class=\"kt-divider\" style=\"border-top-color:#eee;border-top-width:1px;width:80%;border-top-style:solid\"\/><\/div><\/div>\n\n\n\n<p>Ram\u00edrez, O. A., C\u00f3rdova, A., Cerda, M., Lobos, P., H\u00e4rtel, S., Couve, A., &amp; Hidalgo, C.<\/p>\n\n\n\n<p><strong>ABSTRACT<\/strong><br>Neuronal Ca2+ signals are fundamental for synaptic transmission and activity-dependent changes in gene expression. Voltage-gated Ca2+ channels and N-methyl-d-aspartate receptors play major roles in mediating external Ca2+ entry during action potential firing and glutamatergic activity. Additionally, the inositol-1,4,5-trisphosphate receptor (IP3R) and the ryanodine receptor (RyR) channels expressed in the endoplasmic reticulum (ER) also contribute to the generation of Ca2+ signals in response to neuronal activity. The ER forms a network that pervades the entire neuronal volume, allowing intracellular Ca2+ release in dendrites, soma and presynaptic boutons. Despite its unique morphological features, the contributions of ER structure and of ER-shaping proteins such as atlastin &#8211; an ER enriched GTPase that mediates homotypic ER tubule fusion &#8211; to the generation of Ca2+ signals in dendrites remains unreported. Here, we investigated the contribution of RyR-mediated Ca2+ release to IP3-generated Ca2+ signals in dendrites of cultured hippocampal neurons. We also employed GTPase activity-deficient atlastin-2 (ATL2) mutants to evaluate the potential role of atlastin on Ca2+ signaling and ER-resident Ca2+ channel distribution. We found that pharmacological suppression of RyR channel activity increased the rising time and reduced the magnitude and propagation of IP3-induced Ca2+ signals. Additionally, ATL2 mutants induced specific ER morphological alterations, delayed the onset and increased the rising time of IP3-evoked Ca2+ signals, and caused RyR2 and IP3R1 aggregation and RyR2 redistribution. These results indicate that both RyR and ATL2 activity regulate IP3-induced Ca2+ signal dynamics through RyR-mediated Ca2+-induced Ca2+ release, ER shaping and RyR2 distribution.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><a href=\"https:\/\/doi.org\/10.1016\/j.ceca.2021.102399\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.1016\/j.ceca.2021.102399<\/a><br><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ram\u00edrez, O. A., C\u00f3rdova, A., Cerda, M., Lobos, P., H\u00e4rtel, S., Couve, A., &#038; Hidalgo, C. (2021). Ryanodine receptor-mediated Ca2+ release and atlastin-2 GTPase activity contribute to IP3-induced dendritic Ca2+ signals in primary hippocampal neurons. Cell Calcium, 96, 102399.<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[9,1],"tags":[],"class_list":["post-3597","post","type-post","status-publish","format-standard","hentry","category-publications-2021","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/3597","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/comments?post=3597"}],"version-history":[{"count":4,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/3597\/revisions"}],"predecessor-version":[{"id":3613,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/3597\/revisions\/3613"}],"wp:attachment":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/media?parent=3597"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/categories?post=3597"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/tags?post=3597"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}