{"id":3951,"date":"2023-05-17T18:32:19","date_gmt":"2023-05-17T18:32:19","guid":{"rendered":"https:\/\/scian.cl\/scientific-image-analysis\/?p=3951"},"modified":"2023-05-23T15:01:23","modified_gmt":"2023-05-23T15:01:23","slug":"ultraviolet-exposure-of-thymocytes-selective-inhibition-of-apoptosis","status":"publish","type":"post","link":"https:\/\/scian.cl\/scientific-image-analysis\/ultraviolet-exposure-of-thymocytes-selective-inhibition-of-apoptosis\/","title":{"rendered":"Ultraviolet exposure of thymocytes: selective inhibition of apoptosis"},"content":{"rendered":"\n<hr class=\"wp-block-separator has-css-opacity is-style-default\"\/>\n\n\n\n<p>International journal of radiation biology, 80(6), 445-450.<\/p>\n\n\n\n<p>Ojeda, F., Guarda, M. I., Lovengreen, C., Hidalgo, M. A., Folch, H., H\u00e4rtel, S., &amp; Maldonado, C. (2004).<\/p>\n\n\n\n<p><strong>ABSTRACT<\/strong><br><em>Purpose<\/em>: To evaluate selective effects of ultraviolet (UV) irradiation on spontaneous and induced apoptosis in freshly extracted mice thymocytes.<\/p>\n\n\n\n<p><em>Materials and methods<\/em>: Cells were exposed to UV radiation with emission peaks of 365\u2009nm (UVA) exposures of 1620\u201310\u2009200\u2009J\u2009m<sup>\u22122<\/sup>, of 312\u2009nm (UVB) exposures of 34\u20131620\u2009J\u2009m<sup>\u22122<\/sup>&nbsp;or of 254\u2009nm (UVC) exposures of 1.5\u20131620\u2009J\u2009m<sup>\u22122<\/sup>, and incubated for 5.5\u2009h with or without hydrocortisone, phorbol\u201012\u2010myristate\u201013\u2010acetate or anti\u2010Fas antibody. Additionally, cells were irradiated with gamma\u2010rays (5\u2009Gy) before UVB exposure (408\u2009J\u2009m<sup>\u22122<\/sup>) at different times. Apoptosis was quantified by DNA fragmentation.<\/p>\n\n\n\n<p><em>Results<\/em>: Up to an irradiation of 5000\u2009J\u2009m<sup>\u22122<\/sup>, UVA exposure did not show any effect on thymocyte apoptosis, while at 10\u2009200\u2009J\u2009m<sup>\u22122<\/sup>&nbsp;irradiation, considerable DNA fragmentation was observed. In contrast, UVB and UVC irradiation clearly inhibited natural and cortisone\u2010induced apoptosis. Moreover, UVB inhibited apoptosis triggered by phorbol\u201012\u2010myristate\u201013\u2010acetate and gamma\u2010irradiation, but not by anti\u2010Fas antibody.<\/p>\n\n\n\n<p><em>Conclusions<\/em>: The response of mouse thymocytes in culture to UV irradiation strongly depends on the wavelength used. It is suggested that either a survival or an apoptotic pathway occurs depending on the physiological state of the cell, spectral composition of the UV light and cell type. The possible involvement of extracellular signal\u2010regulated kinase and stress\u2010activated protein kinase\/c\u2010Jun N\u2010terminal kinase in the apoptotic pathway is discussed.<\/p>\n\n\n\n<p><a href=\"http:\/\/doi.org\/10.1080\/09553000410001702328\">http:\/\/doi.org\/10.1080\/09553000410001702328<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ojeda, F., Guarda, M. I., Lovengreen, C., Hidalgo, M. A., Folch, H., H\u00e4rtel, S., &#038; Maldonado, C. (2004). Ultraviolet exposure of thymocytes: selective inhibition of apoptosis. International journal of radiation biology, 80(6), 445-450.<\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[66],"tags":[],"class_list":["post-3951","post","type-post","status-publish","format-standard","hentry","category-publications-2004"],"_links":{"self":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/3951","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/comments?post=3951"}],"version-history":[{"count":3,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/3951\/revisions"}],"predecessor-version":[{"id":3994,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/posts\/3951\/revisions\/3994"}],"wp:attachment":[{"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/media?parent=3951"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/categories?post=3951"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scian.cl\/scientific-image-analysis\/wp-json\/wp\/v2\/tags?post=3951"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}