Santiago de Chile 14-29 de Enero 2013, Facultad de Medicina, U-Chile


Miguel Concha, LEO-Lab, U-Chile
Steffen Härtel, SCIAN-Lab, U-Chile


This practical and theoretical course is aimed at graduate students from Latin America interested in the use of optics and microscopic techniques for in vivo 3D visualisation and analysis of cell and tissue dynamics.


Facultad de Medicina, Independencia 1027, U-Chile, Santiago

Profesores Participantes

. Roberto Bernal – (U. Santiago, Chi)
. Miguel Concha – (U. Chile, Chi)
. Mauricio Cerda – (U. Chile, Chi)
. Jorg Enderlein – (U. Gottingen, Ger)
. Nikta Fakhri – (U. Gottingen, Ger)
. Steffen Härtel – (U. Chile, Chi)
. Carl-Philipp Heisenberg – (IST, Austria)
. Jorge Jara – (U. Chile, Chi)
. Ulrich Kubitscheck – (U. Bonn, Ger)
. Omar Ramírez – (U. Chile, Chi)
. Florian Rehfeldt – (U. Gottingen, Ger)
. German Reig – (U. Chile, Chi)
. Felipe Santibañez – (U. Chile, Chi)
. Christoph Schmidt – (U. Gottingen, Ger)
. Jan Spille – (U. Bonn, Ger)
. Juan Pablo Staforelli – (CEFOP, Chi)


. Abigail Díaz – (Mexico)
. Paola Lepanto – (Uruguay)
. Cecilia Plavan – (Uruguay)
. Rodolfo Ortiz – (Argentina)
. Talita Pereira – (Brazil)
. Verónica Akle – (Colombia)
. Juan Ulloa – (Chile)
. Álvaro Glavic – (Chile)
. Magdalena Esparza – (Chile)
. Ricardo Figueroa – (Chile)
. Patricio Olguín – (Chile)
. Verónica Bahamondes – (Chile)

Tópicos centrales:

. Development of zebrafish and annual fish
. Confocal and spinning microscopy
. Light-sheet microscopy
. Super-resolution microscopy
. Photoactivation and laser ablation
. In vivo electroporation
. Force estimation in cells & tissues
. Optical tweezers
. Particle tracking
. Image processing and analysis

Programa y Poster

Download program
Download Day by Day program
Download poster

Literatura para Seminarios

  1. Super-Resolution Microscopy:
    Nanoscopy by Using Blinking Enhanced Quantum Dots PDF Document
    Superresolution imaging for neuroscience PDF Document
    Widely accessible method for superresolution fluorescence imaging of living systems PDF Document
  2. Forces and Microscopy:
    Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells PDF Document
    a-Catenin as a tension transducer that induces adherens junction development PDF Document
    Deconstructing the Cadherin-Catenin-Actin Complex PDF Document
  3. Differentiation of adult stem cells guided by matrix mechanics:
    Cell responses to the mechanochemical microenvironment�Implications for regenerative medicine and drug delivery PDF Document
    Hyaluronic acid matrices show matrix stiffness in 2D and 3D dictates cytoskeletal order and myosin-II phosphorylation within stem cells PDF Document
    Matrix Elasticity Directs Stem Cell Lineage Specification PDF Document
    Supplemental Literature: Cell locomotion and focal adhesions are regulated by substrate flexibility PDF Document
  4. Image Processing:
    Measurement of Instantaneous Velocity Vectors of Organelle Transport: Mitochondrial Transport and Bioenergetics in Hippocampal Neurons PDF Document
    Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy PDF Document (supplementary material ) PDF Document
    3-D Active Meshes Fast Discrete Deformable Models for Cell Tracking in 3-D Time-Lapse Microscopy.pdf PDF Document

Maps and Places

Download PDF here

Créditos / Credits:

Créditos para el curso se otorgan por la Facultad de Medicina, Universidad de Chile. Las materias podrán ser reconocidas para futuros estudios de postgrado, Diplomado y Magíster en Health Informatics.

Aplicación / Application

Deadline for Application and Fellowship – December 28th 2012
Send CV, letter of intention and references from supervisor/mentor
to Miguel Concha and Steffen Härtel

Manuals & More

ZEISS Principles of Confocal Microscopy PDF Document
Principles of Fluorescence Spectroscopy PDF Document
LEICE TCS LSI Brochure PDF Document
Huygens Professional User Guide from SVI: PDF Document Web Site
Intracellular Fluorescent Probe Concentrations by Confocal Microscopy, Finck et al. 1998 PDF Document
Seeing is believing? Alison J. North, The Journal of Cell Biology, Vol. 172, No. 1, January 2, 2006 9�18 PDF Document
The Good, the Bad and the Ugly ! Helen Pearson, NATURE, 447, May 2007 PDF Document
Measuring and interpreting point spread functions to determine confocal microscope resolution and ensure quality control, Cole et al 2011, NATURE PROTOCOL PDF Document
IDL/ScianTimeCalc: 1er Manual de Reconstrución 3D Word Document


Day 1
Clase 1 G Reig PDF Document
Clase 1 S Härtel PDF Document
Clase 1: M Concha PDF Document

Day 2
Clase 2 S Härtel PDF Document
Clase 1 U Kubischeck PDF Document
Clase 3 S Härtel PDF Document

Day 3
Clase 1 J Jara PDF Document
Clase 1 J Spille PDF Document

Day 4
Clase 2 U Kubischeck PDF Document

Day 5 (Friday)
Clase 2 U Kubischeck PDF Document

Day 6 (Monday)
Clase 2 G Reig PDF Document
Clase 2 J Jara PDF Document
Clase 1 R Bernal PDF Document
Clase 2 R Bernal PDF Document

Day 7 (Tuesday)
Clase 1 M Cerda PDF Document

Day 8 (Wednesday)

Day 9 (Thursday)
Clase 2 M Cerda PDF Document


Practico 1 M Cerda PDF Document
Practico 2 M Cerda PDF Document