PUBLICATIONS
2022
Gliovascular alterations in sporadic and familial Alzheimer’s disease: APOE3 Christchurch homozygote glioprotection
Henao‐Restrepo, J., López‐Murillo, C., Valderrama‐Carmona, P., Orozco‐Santa, N., Gomez, J., Gutiérrez‐Vargas, J., … & Posada‐Duque, R. (2022). Gliovascular alterations in sporadic and familial Alzheimer’s disease: APOE3 Christchurch homozygote glioprotection. Brain Pathology, e13119.
Datos de interés público para informar, tomar decisiones y crear conocimiento
Araos R, Basso L, Bravo L, Escobar MJ, Etcheverry A, Härtel S, Hermosilla MP, Jara A, Maass A, Marquet P and Pérez-Acle T (2022). Datos de interés público para informar, tomar decisiones y crear conocimiento. Comité Asesor Ministerial de Datos de Interés Público – MinCiencia
The Dendritic Ergic: Microtubule And Actin Cytoskeletons Participate In Stop‐And‐Go Movement Of Mobile Carriers Between Stable Structures
de los Ángeles Juricic Urzúa, M., Rojas, J. G., Correa, A. C., Cerda, M., Gründler, S. H., & González‐Silva, C. (2022). The dendritic ERGIC: Microtubule and actin cytoskeletons participate in stop‐and‐go movement of mobile carriers between stable structures. Traffic, 23(3), 174-187.
2021
B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction
Ulloa, R., Corrales, O., Cabrera-Reyes, F., Jara-Wilde, J., Saez, J. J., Rivas, C., … & Diaz-Muñoz, J. (2021). B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction. Frontiers in Immunology, 12, 801164-801164.
Mesa técnica “Fondo de Salud Extraordinario: Propuestas de Universidades, Sociedades Científicas y Colegios Profesionales”
Álvarez Román, F.; Araya Saldivar, L.; Varas Rocco, M.; Arribada Maldonado, A.,….Lampert Grassi, MP. (2021). Mesa técnica “Fondo de salud extraordinario : Propuestas de Universidades, Sociedades Científicas y Colegios Profesionales”. Biblioteca del Congreso Nacional de Chile, pp. 245-263.
Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism
Pulgar E., Schwayer C., Guerrero N., López L., Márquez S, Härtel S., Soto R., Heisenberg CP. And ML Concha (2021) Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. eLife 10:e66483
Content‐Based Medical Image Retrieval and Intelligent Interactive Visual Browser for Medical Education, Research and Care
Sotomayor C., Mendoza M., Castañeda V., Farías H., Molina G., Pereira G., Härtel S., Solar M., Araya M. (2021). Content‐Based Medical Image Retrieval and Intelligent Interactive Visual Browser for Medical Education, Research and Care. Diagnostics 2021, 11, 1470.
Ryanodine receptor-mediated Ca2+ release and atlastin-2 GTPase activity contribute to IP3-induced dendritic Ca2+ signals in primary hippocampal neurons
Ramírez, O. A., Córdova, A., Cerda, M., Lobos, P., Härtel, S., Couve, A., & Hidalgo, C. (2021). Ryanodine receptor-mediated Ca2+ release and atlastin-2 GTPase activity contribute to IP3-induced dendritic Ca2+ signals in primary hippocampal neurons. Cell Calcium, 96, 102399.
A global view of standards for open image data formats and repositories.
Swedlow, J.R., Kankaanpää, P., Sarkans, U. et al. A global view of standards for open image data formats and repositories. Nat Methods (2021).
The incidence of psoriasis in Chile: an analysis of the national Waiting List Repository.
Lecaros, C., Dunstan, J., Villena, F., Ashcroft, D. M., Parisi, R., Griffiths, C. E. M., … & De la Cruz, C. (2021). The incidence of psoriasis in Chile: an analysis of the national Waiting List Repository. Clinical and Experimental Dermatology.
RyR-mediated Ca2+ Release Induced by Neuronal Stimulation Participates in Nuclear Ca2+ Signal Generation, CREB Phosphorylation and Npas4 Expression.
Lobos, P., Córdova, A., Vega-Vásquez, I., Ramírez, O. A., Adasme, T., Toledo, J., … & Hidalgo, C. (2021). RyR-mediated Ca2+ release elicited by neuronal activity induces nuclear Ca2+ signals, CREB phosphorylation, and Npas4/RyR2 expression. Proceedings of the National Academy of Sciences, 118(33).
Salmonella Typhimurium Triggers Extracellular Traps release in Murine Macrophages.
Mónaco, A., Canales-Huerta, N., Jara-Wilde, J., Härtel, S., Chabalgoity, J. A., Moreno, M., & Scavone, P. (2021). Salmonella Typhimurium triggers Extracellular Traps release in murine macrophages. Frontiers in cellular and infection microbiology, 11.
Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration.
Alhashem, Z., Portillo, M. A. G., Htun, M. R., Gauert, A., Montecinos, L. B., Härtel, S., & Linker, C. (2021). Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration. In The Epithelial-to Mesenchymal Transition (pp. 79-106). Humana, New York, NY.
2020
Una mirada a la cultura de datos en Chile – Desafíos y Lecciones de la Pandemia por COVID-19.
Submesa de Datos Covid-19, MinCiencia Arancibia D, Araos R, Basso L, Bravo L, Guridi J, Härtel S, Jara J, Maass A, Marquet P, Mora S, & Pérez-Acle T Download PDF
Eosinophils Control Liver Damage by Modulating Immune Responses Against Fasciola hepática.
Frigerio, S., Da Costa, V., Costa, M., Festari, M. F., Landeira, M., Rodríguez-Zraquia, S. A., … & Freire, T. (2020). Eosinophils Control Liver Damage by Modulating Immune Responses Against Fasciola hepatica. Frontiers in Immunology, 11, 2276.
dSTORM microscopy evidences in HeLa cells clustered and scattered γH2AX nanofoci sensitive to ATM, DNA-PK, and ATR kinase inhibitors.
Liddle, P., Jara-Wilde, J., Lafon-Hughes, L., Castro, I., Härtel, S., & Folle, G. (2020). dSTORM microscopy evidences in HeLa cells clustered and scattered γH2AX nanofoci sensitive to ATM, DNA-PK, and ATR kinase inhibitors. Molecular and Cellular Biochemistry, 473(1), 77-91.
Mathematical Modeling for 2D Light–Sheet Fluorescence Microscopy image reconstruction.
Cueva, E., Courdurier, M., Osses, A., Castañeda, V., Palacios, B., & Härtel, S. (2020). Mathematical modeling for 2D light-sheet fluorescence microscopy image reconstruction. Inverse Problems, 36(7), 075005.
Evaluating Online Consumer Medication Information Systems: Comparative Online Usability Study.
Sigle, S., Barriga, P., Fernández, F. J. C., Juhra, C., Härtel, S., & Fegeler, C. (2020). Evaluating Online Consumer Medication Information Systems: Comparative Online Usability Study. JMIR mHealth and uHealth, 8(6), e16648.
Optimising adjacent membrane segmentation and parameterisation in multi‐cellular aggregates by piecewise active contours.
Journal of Microscopy Jara‐Wilde J, Castro I, Lemus Carmen Gloria, Palma Karina, Valdés Francisca, Castañeda Víctor, Hitschfeld Nancy, Concha Miguel L and Steffen Hartel ABSTRACTIn fluorescence microscopy imaging, the segmentation of adjacent cell membranes within…
2019
Evaluating Online Consumer Medication Information Systems: A Comparative Online Usability Study
Stefan Sigle, Pilar Barriga, Francisco Correa, Christian Juhra, Steffen Härtel, Christian Fegeler. ABSTRACTMedication is the most common intervention in Health Care and the number of Online Consumer Information Systems (OCMIS) within the pharmaceutihttps://preprints.jmir.org/preprint/16648cal sector are…
Predicting nationwide obesity from food sales using machine learning. Health Informatics Journal
Jocelyn Dunstan, Marcela Aguirre, Magdalena Bastías, Claudia Nau, Thomas A Glass, Felipe Tobar. LINK:https://doi.org/10.1177/1460458219845959
Chronic Fluoxetine Treatment Induces Maturation-Compatible Changes in the Dendritic Arbor and in Synaptic Responses in the Auditory Cortex
Estibaliz Mariela Ampuero, Mauricio Cerda, Steffen Hartel, Francisco Javier Rubio, Solange Massa, Paula Cubillos, Lorena Abarzua, Rodrigo Sandoval, Ursula Wyneken. LINK:https://doi.org/10.3389/fphar.2019.00804
Is Banning Texturized Implants to Prevent Breast Implant-Associated Anaplastic Large Cell Lymphoma (BIA-ALCL) a Rational Decision? A Meta-Analysis and Cost-Effectiveness Study
Aesthetic Surgery Journalgy. Danilla SV, Jara RP, Miranda F, Bencina F, Aguirre M, Troncoso E, Erazo CA1, Andrades PR, Sepulveda SL, Albornoz CR. LINK:https://doi.org/10.1093/asj/sjz343
Prolonged wait time is associated with increased mortality for Chilean waiting list patients with non-prioritized conditions
BMC Public Health. 2019 Feb 26;19(1):233. doi: 10.1186/s12889-019-6526-6 Martinez DA, Zhang H, Bastias M, Feijoo F, Hinson J, Martinez R, Dunstan J, Levin S, Prieto D. LINK:https://doi.org/10.1186/s12889-019-6526-6
Neuromuscular control during gait in people with haemophilic arthropathy
Cruz-Montecinos C, Pérez-Alenda S, Cerda M, Maas Huub. Haemophilia, 25(2):e69-e77 LINK:https://doi.org/10.1111/hae.13697
Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate
Scientific Reports, 9(1):1835 (2019) Araya C, Hakkinen H, Carcamo L, Cerda M, Savy T, Rookyard C, Peyriéras N, Clarke J. LINK:https://doi.org/10.1186/s12889-019-6526-6
Does epimuscular myofascial force transmission occur between the human quadriceps muscles in vivo during passive stretching?
Journal of Biomechanics, 83:91-96 (2019) Freitas SR, Antunes A, Salmon P, Mendes B, Firmino T, Cruz-Montecinos C, Cerda M, Vaz JR. LINK:https://doi.org/10.3389/fimmu.2020.579801
2018
Assessment of tensile mechanical properties of the Achilles tendon in adult patients with haemophilic arthropathy
Reproducibility study. Haemophilia, 25(1):e27-e29. Cruz-Montecinos C, Pérez-Alenda S, Contreras-Sepúlveda F, Querol F, Cerda M, Maas H. LINK:https://doi.org/10.3389/fimmu.2020.579801
Generation of a HER2 Breast Cancer Gold-Standard Using Supervised Learning from Multiple Experts
Violeta Chang. Lecture Notes in Computer Science, vol 11043, pp 45-54, Springer, doi:10.1007/978-3-030-01364-6_6 ABSTRACTBreast cancer is one of the most common cancer in women around the world. For diagnosis, pathologists evaluate the expression of biomarkers…
A high-speed tracking algorithm for dense granular media
Cerda M, Navaro CA, Silva J, Waitukaitis SR, Mujica N, Hitschfeld N. Computer Physics Communications, doi:10.1016/j.cpc.2018.02.010. LINK:https://doi.org/10.1016/j.cpc.2018.02.010
HER2 Overexpression Segmentation using Machine Learning Classifiers and Quasi-random Patches for Training Data Selection
Raquel Pezoa; Luis Salinas; Claudio Torres; Steffen HärtelApplied Intelligence, submitted
Variable Numbers of Calreticulin Genes in Trypanosoma Cruzi Correlate with Atypical Morphology and Protein Expression
González A, Härtel S, Mansilla J, Sanchez-Valdez F, and Ferreira, AImmunobiology, in press, ref IMBIO51736 LINK:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426574/
Evaluating the 2014 sugar-sweetened beverage tax in Chile: An observational study in urban areas
Ryota Nakamura, Andrew J. Mirelman,Cristóbal Cuadrado,Nicolas Silva-Illanes,Jocelyn Dunstan,Marc Suhrcke – PLOS Medicine Download PDF LINK:https://doi.org/10.1371/journal.pmed.1002596
Honored papers 2017: Gold-standard for computer-assisted morphological sperm analysis, Chang V, Garcia A, Hitschfeld N, Härtel S, Comput Biol Med. 2017 Apr 1; 83:143–150
Edward J. CiaccioIn Press, Accepted Manuscript, Available online 18 May 2018
Honored Papers: Gold-standard for computer-assisted morphological sperm analysis. Computers in Biology and Medicine
Edward J. CiaccioIn Press, Accepted Manuscript, Available online 18 May 2018. LINK:https://doi.org/10.1016/j.compbiomed.2018.05.020
2017
Epigenetic Editing Of The Dlg4/Psd95 Gene Improves Cognition In Aged And Alzheimer’s Disease Mice
Bustos, Fernando J; Ampuero, Estibaliz; Jury, Nur; Aguilar, Rodrigo; Falahi, Fahimeh; Toledo, Jorge; Ahumada, Juan; Lata, Jaclyn; Cubillos, Paula; Henríquez, Berta; Guerra, Miguel V; Stehberg, Jimmy; Neve, Rachael L; Inestrosa, Nibaldo C; Wyneken, Ursula; Fuenzalida,…
Interfering of the reelin/ApoER2/PSD95 signaling axis reactivates structural plasticity of mature hippocampal neurons
Estibaliz Ampuero, Nur Jury, Steffen Härtel, María Paz Marzolo & Brigitte van ZundertJournal of Cellular Physiology, Sep 21. doi: 10.1002/jcp.25605. [Epub ahead of print] ABSTRACTReelin is an extracellular glycoprotein secreted in the embryonic and adult…
Chile’s National Center for Health Information Systems: A Public-Private Partnership to Foster Health Care Information Interoperability
Capurro D, Echeverry A, Figueroa R, Guiñez S, Taramasco C, Galindo C, Avendaño A, García A, Härtel S, doi:10.3233/978-1-61499-830-3-693Stud Health Technol Inform, 245, 693-695. Download PDF LINK:http://ebooks.iospress.nl/publication/48239
Astrocyte-to-neuron communication through integrin-engaged Thy-1/CBP/Csk/Src complex triggers neurite retraction via the RhoA/ROCK pathway
Maldonado H, Calderon C, Burgos-Bravo F, Kobler O, Zuschratter W, Ramirez O, Härtel S, Schneider P, Quest A, Herrera-Molina R & Leyton LBiochim Biophys Acta. 2017 Feb;1864(2):243-254. doi: 10.1016/j.bbamcr.2016.11.006.
Sleep/wake disorders and the hypocretin/orexin system in a zebrafish model of Parkinson’s Disease
Almudena Laliena Izquierdo, Victor Castañeda, Steffen Härtel, Miguel ConchaMechanisms of Development 145, S128, doi.org/10.1016/j.mod.2017.04.352 LINK:https://doi.org/10.1016/j.mod.2017.04.352
CDK5 downregulation enhances synaptic plasticity
Rafael Andrés Posada-Duque, Omar Ramirez, Steffen Härtel, Nibaldo C Inestrosa, Felipe Bodaleo, Christian González-Billault, Alfredo Kirkwood, Gloria Patricia Cardona-GómezCellular and Molecular Life Sciences 74(1), 153-172, doi.org/10.1007/s00018-016-2333-8 LINK:https://doi.org/10.1007/s00018-016-2333-8
Light/dark cycles modulate asymmetric parapineal connectivity to the left habenula in zebrafish larvae
K Palma, M Meynard, V Cornejo, M Cerda, J Jara, S Härtel, M ConchaMechanisms of Development 145, S122-S123, doi.org/10.1016/j.mod.2017.04.334 LINK:https://doi.org/10.1016/j.mod.2017.04.334
Epigenetic editing of the PSD95 gene improves cognition in aged and Alzheimer’s disease mice
Bustos F, Ampuero E, Aguilar R, Jury Nur, Varela-Nallar L, Falahi F, Toledo J, Ahumada J, Lata Jaclyn, Cubillos P, Henríquez B, Guerra M, Stehberg J, Neve R, Inestrosa N, Wyneken U, Fuenzalida M, Härtel…
Asymmetric morphogenesis of the parapineal organ in the embryonic zebrafish brain
Lemus C, Palma K, Jara J, Härtel S and M ConchaMechanisms of Developments, 145, 56, doi:10.1016/j.mod.2017.04.112. LINK:https://doi.org/10.1016/j.mod.2017.04.112
Extra-embryonic tissue spreading directs early embryo morphogenesis in killifish
German Reig, Mauricio Cerda, Néstor Sepúlveda, Daniela Flores, Victor Castaneda, Masazumi Tada, Steffen Härtel, Miguel ConchaNature Communications, 10.1038/ncomms15431 LINK:https://www.nature.com/articles/ncomms15431
Automatic classification of human sperm head morphology
Violeta Chang, Laurent Heutte, Caroline Petitjean, Steffen Härtel, Nancy HitschfeldComputers in Biology and Medicine, 84, 205–216 LINK:http://doi.org/10.1016/j.compbiomed.2017.03.029
RyR-mediated calcium release and mitochondrial ROS generation partake in the synaptic dysfunction caused by amyloid β peptide oligomers
Carol SanMartin, Pablo Veloso, Tatiana Adasme, Alejandra Garcia, Steffen Hartel, Cecilia Hidalgo, Andrea C. Paula-LimaFront. Mol. Neurosci., published Apr 25, doi: 10.3389/fnmol.2017.00115 ABSTRACTThe spreading of mesenchymal-like cell layers is critical for embryo morphogenesis and tissue…
Gold-standard for computer-assisted morphological sperm analysis
Violeta Chang, Alejandra García, Nancy Hitschfeld & Steffen HärtelComputers in Biology and Medicine, 84, 205–216 LINK:http://dx.doi.org/10.1016/j.compbiomed.2017.03.004
Gastrulation in an annual killifish: Molecular and cellular events during germ layer formation in Austrolebias
Luisa Pereiro, Felix Loosli, Juan Fernández, Steffen Härtel, Joachim Wittbrodt, & Miguel ConchaDevelopmental Dynamics, DOI: 10.1002/dvdy.24496 ABSTRACTBackground: Comparative studies beyond the traditional model organisms have been instrumental in enhancing our understanding of the conserved and…
RyR2-mediated Ca2+ release and mitochondrial ROS generation partake in the synaptic dysfunction cause by amyloid B peptide oligomers. Frontiers in Molecular Neuroscience
San Martin C, Veloso Pablo, Adasme T, Lobos P, Bruna B, Galaz J, García A, Härtel S, Hidalgo C and A Paula-LimaISI;SCOPUS/UCH/VID LINK:https://doi.org/10.3389/fnmol.2017.00115
2016
Ultrasound assessment of fascial connec- tivity in the lower limb during maximal cervical flexion: technical aspects and practical application of automatic tracking
Cruz-Montecinos C, Cerda M, Sanzana-Cuche R, Martin-Martin, J. BMC Sports Science, Medicine and Rehabilitation 8:18.
Assessment of Automatically Exported Clinical Data from a Hospital Information System for Clinical Research in Multiple Myeloma
Torres V, Cerda M, Knaup P, Lopprich M. Studies in Health Technology and Informatics 228:332-6.
Segmentation of HER2 protein overexpression in immunohistochemically stained breast cancer images using Support Vector Machines
Raquel Pezoa, Luis Salinas, Claudio Torres, Steffen Hartel, Cristian Maureira-Fredes & Paola ArceJournal of Physics: Conference Series 762 (2016) 012050 / doi:10.1088/1742-6596/762/1/012050 ABSTRACTBreast cancer is one of the most common cancers in women worldwide. Patient…
Directional asymmetry in the volume of the human habenula
Ahumada P, Lemus C, Diaz E, Osorio-Reich M, Härte S and M ConchaBrain Structure and Function, 222(2), 1087-1092, DOI: 10.1007/s00429-016-1231-z ABSTRACTBrain asymmetry is a conserved feature in vertebrates. The dorsal diencephalic habenular complex shows conspicuous…
Leader cells define directionality of trunk, but not cranial, neural crest migration
Richardson J, Gauert A, Briones L, Escudero L, Zainalabdeen A, Gorostiza E, Kabla A, Assar R, Härtel S & C LinkerCell Reports, Cell Reports 15(9), 1-13 ABSTRACTCollective cell migration is fundamental for life and a…
Fimbriae have distinguishable roles in Proteus mirabilis biofilm formation
Scavone P, Iribarnegaray V, Caetano A, Schlapp G, Hartel S, & Zunino PPathogens and Disease: 74(5). pii: ftw033. doi: 10.1093/femspd/ftw033. Epub 2016 Apr 17. ABSTRACTProteus mirabilis is one of the most common etiological agent of…
A frame-based ANN for classification of hyperspectral images: assessment of mechanical damage in mushrooms
Rojas-Moraleda R, Valous N, Gowen A, Esquerre C, Härtel S, Salinas L, & C O’DonnellNeural Computing and Applications (NCAA), pp 1-13, doi:10.1007/s00521-016-2376-7 LINK:https://doi.org/10.1007/s00521-016-2376-7
2015
In vivo relationship between pelvis motion and deep fascia displacement of the medial gastrocnemius: anatomical and functional implications
Cruz-Montecinos C, Gonzalez A, Lopez D, Cerda M, Sanzana-Cuche R, Cuesta-Vargas A. Journal of Anatomy, 227(5):665-72.
Estimation of tensile properties of the Achilles tendon in haemophilic arthropathy of the ankle
Cruz-Montecinos C, Perez-Alenda S, Oyarzun A, Cerda M, Querol F: Case Study. Haemophilia, 21(2):e141e143.
Translational cancer research comes of age in Latin America
Investigators of the U.S. Latin America Cancer Research Network.Science Translational Medicine, Vol. 7(319), pp. 319fs50. doi: 10.1126/scitranslmed.aad5859. ABSTRACTThe U.S. Latin America Cancer Research Network strives to expand translational research capabilities and forge new collaborations. Download…
Higher blood vessel density in comparison to the lymphatic vessels in oral squamous cell carcinoma
Maturana-Ramírez A, Espinoza I, Reyes M, Aitken JP, Aguayo F, Hartel S, & G Rojas-Alcayag.Journal of Clinical and Experimental Pathology, 8(10):13677-86. ABSTRACTIntroduction: Oral squamous cell carcinoma (OSCC) is characterized by local invasion and the development…
An Implementation of Combined Local-Global Optical Flow
Jara J, Cerda M, Delpiano J, and S HärtelImage Processing On Line, 5 (2015), pp. 139�158. ABSTRACTThe optical ow (OF) approaches for motion estimation calculate vector elds which determine the apparent velocities of objects in…
Transactivation activity and nucleocytoplasmic transport of -catenin are independently regulated by its C-terminal end
Maturana J, Niechi I, Silva-Pavez E, Huerta-Castro H, Cataldo R, Härtel S, Barros F, Galindo M & J TapiaGene 573(1), 115�122. http://dx.doi.org/10.1016/j.gene.2015.07.039 ABSTRACTThe key protein in the canonicalWnt pathway is β-catenin, which is phosphorylated both…
Trypanosoma cruzi Infectivity Assessment in ‘in vitro’ Culture Systems by Automated Cell Counting
Liempi A, Castillo C, Cerda M, Droguett D, Duaso J, Barahona K, Hernandez A, Luján C, Fretes R, Härtel S, Kemmerling UActa Tropica, 143:47-50. doi: 10.1016/j.actatropica.2014.12.006 ABSTRACTChagas disease is an endemic, neglected tropical disease in…
Trypanosoma cruzi Calreticulin Topographical Variations in Parasites Infecting Murine Macrophages
González A, Valck C, Sanchez G, Härtel S, Mansilla J, Ramirez G, Fernandez S, Arias JL, Galanti N and Ferreira AThe American Journal of Tropical Medicine and Hygiene, 9, 92(5):887-97. doi: 10.4269/ajtmh.14-0497 ABSTRACTTrypanosoma cruzi calreticulin…
2014
Bleomycin-induced yH2AX foci map preferentially to replicating domains in CHO9 interphase nuclei
Liddle P, Lafon-Hughes L, Di Tomaso MV, Reyes-Ábalos AL, Jara J, Cerda M, Härtel S and G FolleChromosome Research, CHRS-s-13-00133, DOI: 10.1007/s10577-014-9433-9 ABSTRACTExposure to DNA damaging agents triggers phosphorylation of histone variant H2AX (generating γH2AX)…
Gold-standard and improved framework for sperm head segmentation
Chang V, Saavedraa J, Castaneda V, Sarabia L, Hitschfeld N and S HärtelComputer Methods and Programs in Biomedicine, DOI:10.1016/j.cmpb.2014.06.018 ABSTRACTSemen analysis is the first step in the evaluation of an infertile couple. Within this process,…
Computational Methods for Analysis of Dynamic Events In Cell Migration
Castañeda V, Cerda M, Santibañez F, Jara J, Pulgar E, Palma K, Lemus CG, Concha M, Härtel SCurrent Molecular Medicine, 14(2), 291-307. ABSTRACTCell migration is a complex biological process that involves changes in shape and…
Spermatozoa from mice deficient in Niemann-Pick disease type C2 (NPC2) protein have defective cholesterol content and reduced in vitro fertilising ability
Busso D, Oñate-Alvarado MJ, Balboa E, Castro J, Lizama C, Morales G, Vargas S, Härtel S, Moreno RD and S ZanlungoReproduction, Fertility and Development, 26(4), 609-21. doi: 10.1071/RD12059 ABSTRACTThe cholesterol content of the sperm membrane…
Ryanodine receptor-mediated Ca2+ release underlies iron-induced mitochondrial fission and stimulates mitochondrial Ca2+ uptake in primary hippocampal neurons
San Martín CD, Paula-Lima AC, García A, Härtel S, Núñez MT and C HidalgoFrontiers in Molecular Neuroscience, 11, 7-13. doi: 10.3389/fnmol.2014.00013 ABSTRACTMounting evidence indicates that iron accumulation impairs brain function. We have reported previously that…
Optimización y paralización de un algoritmo de generación de skeletons
Ivan Rojas, Memoria de Titulo Para Ingeniero Civil en Computación. UF-Med/FCFM, Universidad de Chile, Santiago, Chile.Tutores: Prof. N Hitschfeld, M Cerda and S Härtel
2013
A Comprehensive System for Facial Animation of Generic 3D Head Models driven by Speech
Terissi L, Cerda M, Gomez JC, Hitschfeld-Kahler N, and Girau B.EURASIP Journal of Audio, Speech and Music Processing (Springer), 5, 5 doi:10.1186/1687-4722-2013-5 ABSTRACTA comprehensive system for facial animation of generic 3D head models driven by…
SOFI of GABAB neurotransmitter receptors in hippocampal neurons elucidates intracellular receptor trafficking and assembly. Proceedings of SPIE.
Huss A, Ramírez OA, Santibañez F, Couve A, Härtel S, Enderlein J. Proceedings of SPIE. Vol. 8590. doi: 10.1117/12.2006215
Stereo Time-of-Flight with Constructive Interference
Victor Castaneda, Diana Mateus and Nassir NavabIEEE Transactions on Pattern Analysis and Machine Intelligence (Accepted) ABSTRACTThis paper describes a novel method to acquire depth images using a pair of ToF? (Time of Flight) cameras. As…
Daam1 is an effector of asymmetric brain morphogenesis that regulates dendritic and axonal outgrowth in dorsal habenular neurons
Colombo A, Palma K, Armijo L, Mione M, Signore IA, Morales C, Guerrero N, Pérez R, Suazo J, Marcelain K, Briones L, Härtel S, Wilson SW, and M ConchaDevelopment, 140, 3997-4007 ABSTRACTAlthough progress has been…
Asymmetry in neural fields: a spatiotemporal encoding mechanism
Cerda M and Girau BBiological Cybernetics Volume 107, Issue 2 (2013), Page 161-178 (Springer). doi:10.1007/s00422-012-0544-0 (Online) ABSTRACTNeural field models have been successfully applied to model diverse brain mechanisms like visual attention, motor control and memory….
2012
Ciencia Computacional Aplicada a la Biomedicina
Jara J, Histchfeld N, and Härtel Sfurther authors : Lemus C, Santibáñez F, Ramirez O, Concha M. Revista BITS de Ciencia, Departamento de Ciencias de la Computacion, Universidad de Chile, Issue 8, pp: 36-43. Download…
TRP, TRPL and cacophony channels mediate Ca2+ influx and exocytosis in axonal terminals of Drosophila photoreceptors
Astorga G, Härtel S, Sanhueza M and J BacigalupoPLosOne, PLoS ONE 7(8): e44182. doi:10.1371/journal.pone.0044182 ABSTRACTIn Drosophila photoreceptors Ca2+-permeant channels TRP and TRPL are the targets of phototransduction, occurring in photosensitive microvilli mediated by a phospholipase…
Generación de skeletons a partir de mallas de superficie
Liliana Alcayaga, Memoria para: Ingeniero Civil en Computación, F-Med/FCFM, Universidad de Chile, Santiago, Chile. Tutores: S Hartel (F-Med) / N Hitschfeld (DCC/FCFM) Download PDF
Performance of optical flow techniques for motion analysis of fluorescent point signals in confocal microscopy
Delpiano J, Jara J, Scheer J, Ramírez O, Ruiz-del-Solar J and S HärtelMachine Vision and Applications, Volume 23, Issue 4, pp: 675-89 ABSTRACTOptical flow (OF) approaches calculate vector fields which determine the apparent velocities of…
Caveolin-1-Enhanced Motility and Focal Adhesion Turnover Require Tyrosine-14 but not Accumulation to the Rear in Metastatic Cancer Cells
Urra H, Torres V, Ortiz R, Lobos L, Díaz M, Díaz N, Härtel S, Leyton L, and A QuestPLosOne, Volume 7, Issue 4, e33085, 1-14 ABSTRACTCaveolin-1 is known to promote cell migration, and increased caveolin-1…
A Method for Analysis of Lipid Vesicle Domain Structure from Confocal Image Data
Husen P, Fidorra M, Härtel S, Bagatolli L, and J IpsenEuropean Biophysics Journal (EBJO), 41(2): 161-75 ABSTRACTQuantitative characterization of the lateral structure of curved membranes based on fluorescence microscopy requires knowledge of the fluorophore distribution…
2011
Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation
Tortosa E, Montenegro-Venegas C, Benoist M, Hartel S, Gonzalez-Billault C, Esteban J and J AvilaJ Biol Chem. 286(47), pp 40638-48. doi: 10.1074/jbc.M111.271320 ABSTRACTMicrotubule-associated protein 1B (MAP1B) is prominently expressed during early stages of neuronal development…
Development of 3D Architecture of Uropathogenic Proteus mirabilis Batch Culture Biofilms – A Quantitative Confocal Microscopy Approach –
Schlapp G, Scavone P, Zunino P and S HärtelJ Microbiol Methods, 87(2) : 234-40 ABSTRACTThis work studies the development of the 3D architecture of batch culture P. mirabilis biofilms on the basis of morpho-topological descriptors…
Changes in Rab3D expression and distribution in acini of Sjögren’s Syndrome patients is associated to loss of cell polarity and secretory dysfunction
Bahamondes V, Albornoz M, Aguilera S, Alliende C, Molina C , Castro I, Urzua U, Quest A, Barrera M, González S, Sanchez M, Härtel S, Hermoso M, Leyton C & González MArthritis & Rheumatism, 63(10):…
Association of age with sperm DNA fragmentation
Aumento del daño en el DNA espermatico en varones mayores de 40 años.
Horta F, Madariaga M, Garcia A, Härtel S & Smith RRevista Médica de Chile, 139: 306-312 ABSTRACTBackground: There is an association between aging and an increased number of sperms with alterations in nuclear DNA. Aim:…
Location matters: the endoplasmic reticulum and protein trafficking in dendrites.
Ramirez O, S Härtel & Couve ABiological Reseach 44: 17-23 ABSTRACTNeurons are highly polarized, but the trafficking mechanisms that operate in these cells and the topological organization of their secretory organelles are still poorly understood….
High-frequency Field Stimulation of Primary Neurons Enhances Ryanodine Receptor-mediated Ca2+ Release and Generates Hydrogen Peroxide, which Jointly Stimulate NF-kB Activity
Riquelme D, Alvarez A, Leal N, Adasme T, Espinoza I, Valdés JA, Troncoso N, Härtel S, Hidalgo J, Hidalgo C, & MA CarrascoAntioxid Redox Signal. 14(7):1245-59 ABSTRACTAbstract Neuronal electrical activity increases intracellular Ca(2+) concentration and…
2010
Confined Displacement Algorithm Determines True and Random Colocalization in Fluorescence Microscopy
Ramirez O, García A, Rojas R, Couve A & S HärtelJournal of Microscopy, Sep 1;239(3):173-83 ABSTRACTThe quantification of colocalizing signals in multi-channel fluorescence microscopy images depends on the reliable segmentation of corresponding regions of interest…
The action of sphingomyelinase in lipid monolayers as revealed by microscopic image analysis
Fanani ML, Härtel S, De Tullio L, Jara J, Olmos F, Oliveira R & B MaggioBiochimica et Biophysica Acta (BBA) – Biomembranes Volume 1798, Issue 7, 1309-1323 ABSTRACTIn recent years, new evidence in biomembrane research…
Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ.
Oteiza P, Koeppen M, Krieg M, Pulgar E, Farias C, Melo C, Preibisch S, Müller D, Tada M, Hartel S, Heisenberg CP, & M ConchaDevelopment, 137:3459-68 Download PDF
Visualization of inositol 1,4,5-trisphosphate receptors on the nuclear envelope outer membrane by deep etching electron microscopy.
Cárdenas C, Escobar M, García A, Osorio-Reich M, Härtel S, Foskett JK, Franzini-Armstrong C.J Struct Biol. 171 (3):372-381 ABSTRACTThe receptors for the second messenger InsP(3) comprise a family of closely related ion channels that release…
2009
Lipid domains in giant unilamellar vesicles and their correspondence with equilibrium thermodynamic phases: A quantitative fluorescence microscopy imaging approach.
Fidorra M, Garcia A, Ipsen J, Härtel S & L Bagatolli.Biochimica et Biophysica Acta (BBA) – Biomembranes, 1788(10):2142-9. [Epub 2009 Aug 21] ABSTRACTWe report a novel analytical procedure to measure the surface areas of coexisting…
Insulin Resistant Skeletal Muscle: Mitochondria Structure, Dynamics And Calcium Homeostasis.
Verónica S Eisner, Alejandra García, Steffen Härtel, Matías Escobar, Mariana Casas, Paola Aedo, David Mears, Marcelo Ezquer, Paulette Conget, Sergio Lavandero, Cecilia Hidalgo, Enrique JaimovichBiophysical Journal, 96(3), 235a, dx.doi.org/10.1016/j.bpj.2008.12.1157 LINK:http://dx.doi.org/10.1016/j.bpj.2008.12.1157
Segmentación de Objetos en Imágenes de Microscopia mediante Contornos Activos con Adaptabilidad Topológica.
Felipe Olmos, Memoria para: Ingeniero Civil en Computación, F-Med/FCFM, Universidad de Chile, Santiago, Chile.Tutores: S Hartel (F-Med) / A Maass (CMM/FCFM) / N Hitschfeld (DCC/FCFM) Download PDF
Dendritic Assembly of Heteromeric Gabab Receptor Subunits in Hippocampal Neurons.
Ramirez O, Vidal R, Tello J, Vargas K, Kindler S, Härtel S & A Couve.J Biol Chem. May 8;284(19):13077-85. [Epub 2009 Mar 10] ABSTRACTUnderstanding the mechanisms that control synaptic efficacy through the availability of neurotransmitter…
SMase-induced domain growth and shape relaxation driven by a rate-dependent, out-of-equilibrium, compositional change.
Fanani ML, De Tullio L, Härtel S, Jara J & B Maggio.Biophysical Journal 96, pp: 67-76. [Epub 2008 Oct 10]. ABSTRACTSphingomyelinase (SMase)-induced ceramide (Cer)-enriched domains in a lipid monolayer are shown to result from an…
NADPH Oxidase and Hydrogen Peroxide Mediate Insulin-induced Calcium Increase in Skeletal Muscle Cells.
Espinosa A, Garcia A, Härtel S, Hidalgo C & E Jaimovich.JBC. 284, pp:2568-2575. [Epub 2008 Nov 20] ABSTRACTSkeletal muscle cells are a main physiological target of insulin. This hormone triggers production of reactive species of…
2008
Changes in mitochondrial dynamics during ceramide-induced cardiomyocyte early apoptosis.
Parra V, Eisner V, Chiong M, Criollo A, Moraga F, Garcia A, Härtel S, Jaimovich E, Zorzano A, Hidalgo C & S Lavandero.Cardiovasc Res. 77(2), pp:387-97. [Epub 2007 Oct 4] ABSTRACTAIMS: In cells, mitochondria are…
Exercise and tachycardia increase NADPH oxidase and ryanodine receptor-2 activity: possible role in cardioprotection.
Sánchez G, Escobar M, Pedrozo Z, Macho P, Domenech R, Härtel S, Hidalgo C, Donoso P.Cardiovasc Res., 77(2), pp:380-6. [Epub 2007 Sep 18] ABSTRACTAIM: Our objective was to investigate in cardiac muscle the contribution of…
2007
Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport.
Vidal RL., Ramírez OA., Sandoval L., Koenig-Robert R., Härtel S. and Couve A.Molecular and Cellular Neuroscience, 35(3):501-12. ABSTRACTThe cytoskeleton and cytoskeletal motors play a fundamental role in neurotransmitter receptor trafficking, but proteins that link GABAB…
The initial surface composition and topography modulate sphingomyelinase-driven sphingomyelin to ceramide conversion in lipid monolayers.
De Tullio L., Maggio B., Härtel S., Jara J. & Fanani ML.Cell Biochemistry and Biophysics 47, pp:169�177. ABSTRACTChanges of the initial composition and topography of mixed monolayers of Sphingomyelin and Ceramide modulate the degradation of…
3D Morpho-Topological Analysis of Asymmetric Neuronal Morphogenesis in Developing Zebrafish. Computational Modelling of Objects Represented in Images. Fundamentals, Methods and Applications.
S. Härtel, J. Jara, C.G. Lemus, M.L. ConchaEd. João Manuel Tavares & Jorge Nata, Taylor and Francis Group, ISBN: 9780415433495, pp: 215-220. ABSTRACTWe applied in vivo confocal microscopy of GFP-transgenic zebrafish in combination with 3D…
2006
Contornos activos en tres dimensiones para la segmentación de estructuras biológicas.
Jorge Jara Wilde, Proyecto de Titulo: INGENIERO CIVIL EN INFORMÁTICAINFORMÁTICA, Universidad Austral de Chile, Valdivia, Chile. ABSTRACTIn the field of image processing, the segmentation techniques allow to identify and to define regions of interest (ROIs)…
Surface-bound phosphatase activity in ectomycorrhizal fungi: a comparative study between a colorimetric and a microscope-based method.
Alvarez M., Godoy R., Gieseke A., & Härtel S.Biology & Fertility of Soils 42(6), 561-568, doi.org/10.1007/s00374-005-0053-6 ABSTRACTFor the quantification of surface bound phosphomonoesterase activity (SBPA) of fungi, roots, or mycorrhiza, a colorimetric method based on…
ATP steal between cation pumps: a mechanism linking Na+ influx to the onset of necrotic Ca2+ overload.
Castro J, Ruminot I, Porras OH, Flores CM, Hermosilla T, Verdugo E, Venegas F, Hartel S, Michea L & Barros LF.Cell Death & Differentiation 13, pp:1675�1685. ABSTRACTWe set out to identify molecular mechanisms underlying the…