PUBLICATIONS

2021

Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism

Pulgar E., Schwayer C., Guerrero N., López L., Márquez S, Härtel S., Soto R., Heisenberg CP. And ML Concha (2021) Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. eLife 10:e66483

Content‐Based Medical Image Retrieval and Intelligent Interactive Visual Browser for Medical Education, Research and Care

Sotomayor C., Mendoza M., Castañeda V., Farías H., Molina G., Pereira G., Härtel S., Solar M., Araya M. (2021). Content‐Based Medical Image Retrieval and Intelligent Interactive Visual Browser for Medical Education, Research and Care. Diagnostics 2021, 11, 1470.

Ryanodine receptor-mediated Ca2+ release and atlastin-2 GTPase activity contribute to IP3-induced dendritic Ca2+ signals in primary hippocampal neurons

Ramírez, O. A., Córdova, A., Cerda, M., Lobos, P., Härtel, S., Couve, A., & Hidalgo, C. (2021). Ryanodine receptor-mediated Ca2+ release and atlastin-2 GTPase activity contribute to IP3-induced dendritic Ca2+ signals in primary hippocampal neurons. Cell Calcium, 96, 102399.

RyR-mediated Ca2+ Release Induced by Neuronal Stimulation Participates in Nuclear Ca2+ Signal Generation, CREB Phosphorylation and Npas4 Expression.

Lobos, P., Córdova, A., Vega-Vásquez, I., Ramírez, O. A., Adasme, T., Toledo, J., … & Hidalgo, C. (2021). RyR-mediated Ca2+ release elicited by neuronal activity induces nuclear Ca2+ signals, CREB phosphorylation, and Npas4/RyR2 expression. Proceedings of the National Academy of Sciences, 118(33).

2020

dSTORM microscopy evidences in HeLa cells clustered and scattered γH2AX nanofoci sensitive to ATM, DNA-PK, and ATR kinase inhibitors.

Liddle, P., Jara-Wilde, J., Lafon-Hughes, L., Castro, I., Härtel, S., & Folle, G. (2020). dSTORM microscopy evidences in HeLa cells clustered and scattered γH2AX nanofoci sensitive to ATM, DNA-PK, and ATR kinase inhibitors. Molecular and Cellular Biochemistry, 473(1), 77-91.

Optimising adjacent membrane segmentation and parameterisation in multi‐cellular aggregates by piecewise active contours.

Journal of Microscopy Jara‐Wilde J, Castro I, Lemus Carmen Gloria, Palma Karina, Valdés Francisca, Castañeda Víctor, Hitschfeld Nancy, Concha Miguel L and Steffen Hartel ABSTRACTIn fluorescence microscopy imaging, the segmentation of adjacent cell membranes within…

2019

Evaluating Online Consumer Medication Information Systems: A Comparative Online Usability Study

Stefan Sigle, Pilar Barriga, Francisco Correa, Christian Juhra, Steffen Härtel, Christian Fegeler. ABSTRACTMedication is the most common intervention in Health Care and the number of Online Consumer Information Systems (OCMIS) within the pharmaceutihttps://preprints.jmir.org/preprint/16648cal sector are…

Chronic Fluoxetine Treatment Induces Maturation-Compatible Changes in the Dendritic Arbor and in Synaptic Responses in the Auditory Cortex

Estibaliz Mariela Ampuero, Mauricio Cerda, Steffen Hartel, Francisco Javier Rubio, Solange Massa, Paula Cubillos, Lorena Abarzua, Rodrigo Sandoval, Ursula Wyneken. LINK:https://doi.org/10.3389/fphar.2019.00804

Is Banning Texturized Implants to Prevent Breast Implant-Associated Anaplastic Large Cell Lymphoma (BIA-ALCL) a Rational Decision? A Meta-Analysis and Cost-Effectiveness Study

Aesthetic Surgery Journalgy. Danilla SV, Jara RP, Miranda F, Bencina F, Aguirre M, Troncoso E, Erazo CA1, Andrades PR, Sepulveda SL, Albornoz CR. LINK:https://doi.org/10.1093/asj/sjz343

2018

2017

Interfering of the reelin/ApoER2/PSD95 signaling axis reactivates structural plasticity of mature hippocampal neurons

Estibaliz Ampuero, Nur Jury, Steffen Härtel, María Paz Marzolo & Brigitte van ZundertJournal of Cellular Physiology, Sep 21. doi: 10.1002/jcp.25605. [Epub ahead of print] ABSTRACTReelin is an extracellular glycoprotein secreted in the embryonic and adult…

Chile’s National Center for Health Information Systems: A Public-Private Partnership to Foster Health Care Information Interoperability

Capurro D, Echeverry A, Figueroa R, Guiñez S, Taramasco C, Galindo C, Avendaño A, García A, Härtel S, doi:10.3233/978-1-61499-830-3-693Stud Health Technol Inform, 245, 693-695. Download PDF LINK:http://ebooks.iospress.nl/publication/48239

Astrocyte-to-neuron communication through integrin-engaged Thy-1/CBP/Csk/Src complex triggers neurite retraction via the RhoA/ROCK pathway

Maldonado H, Calderon C, Burgos-Bravo F, Kobler O, Zuschratter W, Ramirez O, Härtel S, Schneider P, Quest A, Herrera-Molina R & Leyton LBiochim Biophys Acta. 2017 Feb;1864(2):243-254. doi: 10.1016/j.bbamcr.2016.11.006.

RyR-mediated calcium release and mitochondrial ROS generation partake in the synaptic dysfunction caused by amyloid β peptide oligomers

Carol SanMartin, Pablo Veloso, Tatiana Adasme, Alejandra Garcia, Steffen Hartel, Cecilia Hidalgo, Andrea C. Paula-LimaFront. Mol. Neurosci., published Apr 25, doi: 10.3389/fnmol.2017.00115 ABSTRACTThe spreading of mesenchymal-like cell layers is critical for embryo morphogenesis and tissue…

Gastrulation in an annual killifish: Molecular and cellular events during germ layer formation in Austrolebias

Luisa Pereiro, Felix Loosli, Juan Fernández, Steffen Härtel, Joachim Wittbrodt, & Miguel ConchaDevelopmental Dynamics, DOI: 10.1002/dvdy.24496 ABSTRACTBackground: Comparative studies beyond the traditional model organisms have been instrumental in enhancing our understanding of the conserved and…

RyR2-mediated Ca2+ release and mitochondrial ROS generation partake in the synaptic dysfunction cause by amyloid B peptide oligomers. Frontiers in Molecular Neuroscience

San Martin C, Veloso Pablo, Adasme T, Lobos P, Bruna B, Galaz J, García A, Härtel S, Hidalgo C and A Paula-LimaISI;SCOPUS/UCH/VID LINK:https://doi.org/10.3389/fnmol.2017.00115

2016

Segmentation of HER2 protein overexpression in immunohistochemically stained breast cancer images using Support Vector Machines

Raquel Pezoa, Luis Salinas, Claudio Torres, Steffen Hartel, Cristian Maureira-Fredes & Paola ArceJournal of Physics: Conference Series 762 (2016) 012050 / doi:10.1088/1742-6596/762/1/012050 ABSTRACTBreast cancer is one of the most common cancers in women worldwide. Patient…

2015

Transactivation activity and nucleocytoplasmic transport of -catenin are independently regulated by its C-terminal end

Maturana J, Niechi I, Silva-Pavez E, Huerta-Castro H, Cataldo R, Härtel S, Barros F, Galindo M & J TapiaGene 573(1), 115�122. http://dx.doi.org/10.1016/j.gene.2015.07.039 ABSTRACTThe key protein in the canonicalWnt pathway is β-catenin, which is phosphorylated both…

2014

Spermatozoa from mice deficient in Niemann-Pick disease type C2 (NPC2) protein have defective cholesterol content and reduced in vitro fertilising ability

Busso D, Oñate-Alvarado MJ, Balboa E, Castro J, Lizama C, Morales G, Vargas S, Härtel S, Moreno RD and S ZanlungoReproduction, Fertility and Development, 26(4), 609-21. doi: 10.1071/RD12059 ABSTRACTThe cholesterol content of the sperm membrane…

Ryanodine receptor-mediated Ca2+ release underlies iron-induced mitochondrial fission and stimulates mitochondrial Ca2+ uptake in primary hippocampal neurons

San Martín CD, Paula-Lima AC, García A, Härtel S, Núñez MT and C HidalgoFrontiers in Molecular Neuroscience, 11, 7-13. doi: 10.3389/fnmol.2014.00013 ABSTRACTMounting evidence indicates that iron accumulation impairs brain function. We have reported previously that…

2013

2012

TRP, TRPL and cacophony channels mediate Ca2+ influx and exocytosis in axonal terminals of Drosophila photoreceptors

Astorga G, Härtel S, Sanhueza M and J BacigalupoPLosOne, PLoS ONE 7(8): e44182. doi:10.1371/journal.pone.0044182 ABSTRACTIn Drosophila photoreceptors Ca2+-permeant channels TRP and TRPL are the targets of phototransduction, occurring in photosensitive microvilli mediated by a phospholipase…

Caveolin-1-Enhanced Motility and Focal Adhesion Turnover Require Tyrosine-14 but not Accumulation to the Rear in Metastatic Cancer Cells

Urra H, Torres V, Ortiz R, Lobos L, Díaz M, Díaz N, Härtel S, Leyton L, and A QuestPLosOne, Volume 7, Issue 4, e33085, 1-14 ABSTRACTCaveolin-1 is known to promote cell migration, and increased caveolin-1…

2011

Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation

Tortosa E, Montenegro-Venegas C, Benoist M, Hartel S, Gonzalez-Billault C, Esteban J and J AvilaJ Biol Chem. 286(47), pp 40638-48. doi: 10.1074/jbc.M111.271320 ABSTRACTMicrotubule-associated protein 1B (MAP1B) is prominently expressed during early stages of neuronal development…

Development of 3D Architecture of Uropathogenic Proteus mirabilis Batch Culture Biofilms – A Quantitative Confocal Microscopy Approach –

Schlapp G, Scavone P, Zunino P and S HärtelJ Microbiol Methods, 87(2) : 234-40 ABSTRACTThis work studies the development of the 3D architecture of batch culture P. mirabilis biofilms on the basis of morpho-topological descriptors…

Changes in Rab3D expression and distribution in acini of Sjögren’s Syndrome patients is associated to loss of cell polarity and secretory dysfunction

Bahamondes V, Albornoz M, Aguilera S, Alliende C, Molina C , Castro I, Urzua U, Quest A, Barrera M, González S, Sanchez M, Härtel S, Hermoso M, Leyton C & González MArthritis & Rheumatism, 63(10):…

High-frequency Field Stimulation of Primary Neurons Enhances Ryanodine Receptor-mediated Ca2+ Release and Generates Hydrogen Peroxide, which Jointly Stimulate NF-kB Activity

Riquelme D, Alvarez A, Leal N, Adasme T, Espinoza I, Valdés JA, Troncoso N, Härtel S, Hidalgo J, Hidalgo C, & MA CarrascoAntioxid Redox Signal. 14(7):1245-59 ABSTRACTAbstract Neuronal electrical activity increases intracellular Ca(2+) concentration and…

2010

Visualization of inositol 1,4,5-trisphosphate receptors on the nuclear envelope outer membrane by deep etching electron microscopy.

Cárdenas C, Escobar M, García A, Osorio-Reich M, Härtel S, Foskett JK, Franzini-Armstrong C.J Struct Biol. 171 (3):372-381 ABSTRACTThe receptors for the second messenger InsP(3) comprise a family of closely related ion channels that release…

2009

Lipid domains in giant unilamellar vesicles and their correspondence with equilibrium thermodynamic phases: A quantitative fluorescence microscopy imaging approach.

Fidorra M, Garcia A, Ipsen J, Härtel S & L Bagatolli.Biochimica et Biophysica Acta (BBA) – Biomembranes, 1788(10):2142-9. [Epub 2009 Aug 21] ABSTRACTWe report a novel analytical procedure to measure the surface areas of coexisting…

Insulin Resistant Skeletal Muscle: Mitochondria Structure, Dynamics And Calcium Homeostasis.

Verónica S Eisner, Alejandra García, Steffen Härtel, Matías Escobar, Mariana Casas, Paola Aedo, David Mears, Marcelo Ezquer, Paulette Conget, Sergio Lavandero, Cecilia Hidalgo, Enrique JaimovichBiophysical Journal, 96(3), 235a, dx.doi.org/10.1016/j.bpj.2008.12.1157 LINK:http://dx.doi.org/10.1016/j.bpj.2008.12.1157

2008

2007

Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport.

Vidal RL., Ramírez OA., Sandoval L., Koenig-Robert R., Härtel S. and Couve A.Molecular and Cellular Neuroscience, 35(3):501-12. ABSTRACTThe cytoskeleton and cytoskeletal motors play a fundamental role in neurotransmitter receptor trafficking, but proteins that link GABAB…

The initial surface composition and topography modulate sphingomyelinase-driven sphingomyelin to ceramide conversion in lipid monolayers.

De Tullio L., Maggio B., Härtel S., Jara J. & Fanani ML.Cell Biochemistry and Biophysics 47, pp:169�177. ABSTRACTChanges of the initial composition and topography of mixed monolayers of Sphingomyelin and Ceramide modulate the degradation of…

3D Morpho-Topological Analysis of Asymmetric Neuronal Morphogenesis in Developing Zebrafish. Computational Modelling of Objects Represented in Images. Fundamentals, Methods and Applications.

S. Härtel, J. Jara, C.G. Lemus, M.L. ConchaEd. João Manuel Tavares & Jorge Nata, Taylor and Francis Group, ISBN: 9780415433495, pp: 215-220. ABSTRACTWe applied in vivo confocal microscopy of GFP-transgenic zebrafish in combination with 3D…

2006

Surface-bound phosphatase activity in ectomycorrhizal fungi: a comparative study between a colorimetric and a microscope-based method.

Alvarez M., Godoy R., Gieseke A., & Härtel S.Biology & Fertility of Soils 42(6), 561-568, doi.org/10.1007/s00374-005-0053-6 ABSTRACTFor the quantification of surface bound phosphomonoesterase activity (SBPA) of fungi, roots, or mycorrhiza, a colorimetric method based on…